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Cloning and Expression
Cell Culture / Tissue Culture
Cell Culture Reagents
IPTG
IPTG, Dioxane-Free
IPTG, Dioxane-Free from Promega
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Citations:
(6)
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IPTG, Dioxane-Free
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Description
An inducer of beta-galactosidase activity in many bacteria
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
IPTG, Dioxane-Free
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
V3955
Quantity
1g
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (6)
(1)
USP7 regulates the ERK1/2 signaling pathway through deubiquitinating Raf-1 in lung adenocarcinoma
Cell death & disease
August 10, 2022
Park HB, Hwang S, Baek KH.
tube.
Transfected
cells were induced using 1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Cat #
V3955
,
Promega
, Madison, WI, USA) and adjusted to an A600 of 0.4–0.5. Cells were lysed by
sonication
and incubated
(2)
Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis
Nutrients
February 4, 2022
Lin H, Guo X, Liu J, Liu P, Mei G, Li H, Li D, Chen H, Chen L, Zhao Y,
et al
.
ThermoFisher Scientific
(
12566014
). DAPI (C1006) and Hoechst (C1002) were bought from Beyotime. IPTG (
V3955
) and BeadsTM GSH (70601-5) were purchased from
Promega
and
Beaver
. The plasmids of Rab7Wt (W1949Gn),
(3)
Identification of scavenger receptor B1 as the airway microfold cell receptor for
Mycobacterium tuberculosis
eLife
March 5, 2020
Khan HS, Nair VR, Ruhl CR, Alvarez-Arguedas S, Galvan Rendiz JL, Franco LH, Huang L, Shaul PW, Kim J, Xie Y,
et al
.
protein expression
. 1 L of bacterial culture was grown to an OD600 of 0.6, induced with 1 mM IPTG (
Promega
V3955
) at 37˚C for 3 hr, and centrifuged at 3500 rpm for 15 min at 4˚C to yield a bacterial pellet. The bacterial
(4)
Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach
Current Protocols in Chemical Biology
June 22, 2018
Joshua M. Lubner, Jeremy L. Balsbaugh, George M. Church, Michael F. Chou, Daniel Schwartz
(see Reagents and Solutions) Isopropyl β-D-1-thiogalactopyranoside (IPTG) (
Promega
cat. no.
V3951
or
V3955
) Tris Base (
Sigma Aldrich
cat. no. T6066)
Sodium Chloride
(Fisher Scientific cat. no. S671-500)
(5)
Identification of fibroblast growth factor receptor 3 (FGFR3) as a protein receptor for botulinum neurotoxin serotype A (BoNT/A)
PLoS pathogens
January 1, 2013
Jacky BP, Garay PE, Dupuy J, Nelson JB, Cai B, Molina Y, Wang J, Steward LE, Broide RS, Francis J,
et al
.
E.Coli
, Acella Electrocompetent BL21(DE3) (
42649
;
Edge Biosystems
). Expression was induced by 1 mM IPTG (
V3955
;
Promega
) at either 37°C for 16 hours (FGFR3b/c peptides) or at 16°C for 16 hours (rHC/A).
For purification
(6)
The mu-opioid receptor and the NMDA receptor associate in PAG neurons: implications in pain control
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology
January 1, 2012
Rodríguez-Muñoz M, Sánchez-Blázquez P, Vicente-Sánchez A, Berrocoso E, Garzón J.
C0– C2), and NR1 (segments C0–C1–C2)
strains
were grown to an optical density of 0.5–0.6. IPTG (
Promega
; #
V3955
) and Neuropsychopharmacology Association mu-opioid receptor–NMDA receptor M Rodrı́guez-Muñoz et
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IPTG, Dioxane-Free from Promega
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